ABISHEK VENKATARAMAN

Master's Student in Biotechnology & Pharmaceutical Engineering

Pittsburgh, US.

About

Abishek is currently pursuing a Master of Science in Biotechnology and Pharmaceutical Engineering at Carnegie Mellon, with a strong foundation in Chemical Engineering from Penn State. He brings robust research experience in cell therapy, drug development, and microfluidics, demonstrating expertise in aseptic mammalian cell culture, nanoparticle formulation, and computational protein engineering. His profile highlights a blend of wet-lab proficiency and technical skills, positioning him for advanced roles in bioprocess development, pharmaceutical R&D, and biotechnology innovation.

Work

Carnegie Mellon University

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Graduate Researcher at Ren Lab (BSL-2) – Cell Therapy and Tissue Engineering

Pittsburgh, PA, US

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Summary

Led aseptic mammalian cell culture of human retinal epithelial cells for advanced tissue engineering applications, meticulously documenting cell growth and passage.

Highlights

Conducted aseptic mammalian cell culture of human retinal epithelial cells, significantly advancing tissue engineering applications in a BSL-2 environment.

Maintained optimal cell growth through routine cell passaging in T-flasks, meticulously documenting passage numbers and cell counts for experimental integrity and reproducibility.

Carnegie Mellon University

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Graduate Researcher at Niepa microBiointerface Lab (BSL-2) - Cell Culture Development

Pittsburgh, PA, US

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Summary

Engineered and integrated self-fabricated microfluidic devices to encapsulate bacterial strains, enabling high-resolution imaging for microbial growth dynamics.

Highlights

Engineered and integrated self-fabricated droplet-based microfluidic devices to encapsulate *Staphylococcus aureus* in polymeric micro-capsules, enabling high-resolution imaging for microbial growth dynamics.

Achieved a high throughput of 80 bacterial-loaded capsules per second, each with a 25 nanoliter core volume, significantly enhancing experimental scale and efficiency.

Prepared and precisely measured osmolality of sterilized media, including lysogeny broth and UFTYE, ensuring optimal conditions for reliable microbiological testing.

Developed proficiency in overnight bacterial cell culture within culture shake flasks, utilizing sterile aseptic techniques for reliable experimental outcomes.

The Pennsylvania State University

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Undergraduate Researcher at Sheikhi Lab - Drug Development

University Park, PA, US

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Summary

Fabricated monodisperse drug-loaded PLGA microspheres using 3D-printed microfluidic chips for the development of early-stage tissue regenerative drug products.

Highlights

Fabricated monodisperse drug-loaded PLGA (poly lactic-co-glycolic acid) microspheres utilizing 3D-printed droplet-based microfluidic chips, advancing early-stage tissue regenerative drug product development.

Maximized drug product purity through efficient purification of drug-loaded PLGA microspheres via ultracentrifugation and lyophilization processes, ensuring high-quality regenerative drug products.

Aswan International Engineering LLC. (Oil and Gas)

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Industrial Intern

Dubai, Dubai, United Arab Emirates

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Summary

Collaborated with a quality control team to enhance manufacturing processes for corrosion-resistant tungsten carbide coatings on oil and gas downhole tools.

Highlights

Collaborated with quality control engineers to optimize thermal spray technologies, enhancing the manufacturing of corrosion-resistant tungsten carbide coatings for oil and gas downhole tools.

Supported routine QC testing procedures, ensuring adherence to quality standards and operational efficiency for critical industrial components.

Education

Carnegie Mellon University

Pittsburgh, PA, United States of America
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Master of Science Biotechnology and Pharmaceutical Engineering Grade: 3.84/4.00

Courses

Protein Engineering

Bioprocess Design

Synthetic Biology

Molecular Biology Techniques

Structural DNA-Nanotechnology

Bio-separations and Bioprocessing Analytics

Applied Cell and Molecular Biology

Precision Medicine

The Pennsylvania State University

University Park, PA, United States of America
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Bachelor of Science Chemical Engineering and Minor in Energy Engineering Grade: 3.33/4.00

Courses

Process Design

Drug Delivery and Pharmacokinetics

Biomolecular Engineering

Process Heat and Mass Transfer

Chemical Reaction Engineering

Hydrogen and Fuel Cells

Process Safety

Life Cycle Assessment

Awards

Dean's List

Carnegie Mellon University

Awarded for academic excellence during Master's studies.

Dean's List

The Pennsylvania State University

Awarded for academic excellence in Spring 2024 semester.

Dean's List

The Pennsylvania State University

Awarded for academic excellence in Fall 2023 semester.

Dean's List

The Pennsylvania State University

Awarded for academic excellence in Spring 2023 semester.

Languages

English

Skills

Wet-Lab

Cell Culture, Nanoparticle-Formulation, PCR Cloning, Protein Purification, Vector Design, DLS, Zeta Potential, Imaging.

Process-Based

Downstream Purification, Drug Delivery, Bioreactor Design, PFDs, Pharmacokinetic Modeling, HAZOP, RCA.

Technical

Computational Modeling, MATLAB, Scientific Writing, Design of Experiments, ML models, Programming using R.

Projects

Analytical Characterization of mRNA-lipid nanoparticles (mRNA-LNPs)

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Summary

This project focused on the synthesis and characterization of mRNA-lipid nanoparticles, optimizing their formulation for size and encapsulation efficiency.

Computational Protein Engineering - Antibody Drug Discovery (AI-enabled tools)

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Summary

Utilized AI-enabled computational tools to analyze antibody complexes and engineer enhanced binding kinetics for protein-ligand interactions.

Downstream Processing of Lipopeptide Antibiotics (Daptomycin) Presentation

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Summary

Developed comprehensive, industrial-relevant process flow diagrams for the isolation and end-to-end downstream processing of daptomycin.

Synthesis of an anti-GFP monoclonal antibody (Laboratory Course Element)

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Summary

Involved the isolation of plasmids and PCR cloning for the construction and confirmation of a variable light chain antibody region.